BUFFERED EXTRACTION OF HUMULONE ANDISOHUMULONE FROM WORT AND BEER

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Abstract
Worts and beers have been adjusted to pH values ranging from 0·6–7·5 prior to extraction with hydrocarbon solvents. Determinations of absorptions over the range 230–360 mμ. and estimations of the contents of isohumulones, iso-compounds and humulones in the extracts have shown that humulones were extracted over the complete pH range and were differentiated from isohumulones and iso-compounds above pH 5. Conversely, when hydrocarbon extracts were shaken with buffer solutions of varying pH value, as the pH increased, so did the efficiency of removal of isohumulones, etc., increase, with some differentiation of the constituents. With petroleum ether and iso-octane extracts, about 30%, and with chloroform extracts, about 60%, of the total solids could not be accounted for as bittering substances. Chloroform following the hydrocarbons extracted appreciable amounts of unidentified substances. The true distribution coefficient for isohumulones between water and iso-octane was calculated to be about 0·025. Although the theoretical expectations were not fully realized experimentally, there were indications that suitable buffers could be useful for separating bittering substances on a preparative scale.

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