Investigation with Tritiated Thymidine of the Relationship Between the Sex Chromosomes, Sex Chromatin, and the Drumstick in the Cells of the Female Nine-Banded Armadillo, Dasypus novemcinctus

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Abstract
The functional analogies between the sex chromosomes, sex chromatin, and the drumstick in the cells of the female nine-banded armadillo (Dasypus noυemcinctus) were investigated utilizing tritium labelled thymidine both in vitro and in vivo. Tissue culture studies demonstrated that one of the female X chromosomes is asynchronous and synthesizes DNA later than the other X and most of the autosomal complement. In interphase nuclei a peripheral focus of heavier labelling was found overlying the Barr body, indicating that the sex chromatin is formed by a great part or all of the late replicating X chromosome. Localized labelling was also observed over several drumsticks in studies performed in vivo supporting the view that a relationship exists between the drumstick and the asynchronous X chromosome.