A Novel Method for Clean-Up of Coal Tar Dyes Using a Quaternary Amine Cartridge

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Abstract
A simple, rapid, and reliable clean-up method has been developed for identification of the 12 coal tar dyes permitted in Japan by using a quaternary amine cartridge. The dyes were directly extracted from a sample with an appropriate solvent such as water, ethanol, and ammoniacal ethanol without elimination of fat contained in the sample. The extract was directly applied to a Sep-pak QMA cartridge without pH adjustment or elimination of ethanol. The dyes retained on the cartridge were eluted with 3% hydrochloric methanol solution and the eluate was analyzed by high performance liquid chromatography and thin layer chromatography after neutralization of the eluate with aqueous ammonia. Recoveries of the 12 permitted coal tar dyes from white chocolate fortified at levels of 5ppm and 10ppm were 85.8-97.9% and 83.3-95.6%, respectively.

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