Comparison of Amplified Mycobacterium Tuberculosis Direct Test (MTD), Amplicor Mycobacteria kit (Amplicor) and PCR Method for Detection Mycobacterium tuberculosis in Clinical Specimens
- 1 January 1995
- journal article
- research article
- Published by The Japanese Association for Infectious Diseases in Kansenshogaku Zasshi
- Vol. 69 (5) , 539-545
- https://doi.org/10.11150/kansenshogakuzasshi1970.69.539
Abstract
Accuracy amplified Mycobacterium Tuberculosis Direct Test (MTD), Amplicor Mycobacteria kit (Amplicor) and PCR method routinely used in the University of Tokyo Hospital (J. Clin. Microbiol. 31: 446-450 1991) were evaluated and compared with the same samples. The detection limits of MTD, Amplicor and PCR method (University of Tokyo) were 0.01-0.1 CFU/tube, 0.625 CFU/tube and 0.2 CFU/tube respectively, which were almost the same. These were shown to be at least as sensitive as the conventional culture techniques. The Tokyo Univ. method using radioisotope, takes up to 3 days, on the other hand 2 kits take 4-5 hours. These kits become useful tools for the early and rapid detection of M. tuberculosis in uncultured clinical specimens. But the risk of laboratory contamination and false-positive results remain. These must be further improved.Keywords
This publication has 0 references indexed in Scilit: