Cloning of the sporulation gene spoVE in Bacillus subtilis.

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Abstract
The sporulation gene spoVE of B. subtilis was cloned by the prophage transformation method in temperate phage .vphi.105. The specialized transducing phage .vphi.105spoVE, restored the sporulation of the sporulation-defective mutant of B. subtilis strain 1S51 (spoVE85). Transformation experiments showed that the spoVE gene resides on a 1.7 megadalton (md) EcoRI restriction fragment of the transducing phage genome. Subsequently, the 1.7 Mdalton fragment was recloned into the EcoRI site of a plasmid pUB110 and plasmids having a deletion within the 1.7 Mdalton insert were constructed. The plasmid carrying the entire spoVE gene restored the sporulation of strain 5202 (spoVE85 recE4) to a frequency of 103 spores/ml, but inhibited the sporulation of strain 4309 (spo+ recE4) to a level of 104 spores/ml.

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