Controlled Initiation of Chromosomal Replication inEscherichia coliRequires Functional Hda Protein
Open Access
- 15 May 2003
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 185 (10) , 3244-3248
- https://doi.org/10.1128/jb.185.10.3244-3248.2003
Abstract
Regulatory inactivation of DnaA helps ensure that the Escherichia coli chromosome is replicated only once per cell cycle, through accelerated hydrolysis of active replication initiator ATP-DnaA to inactive ADP-DnaA. Analysis of Δhda strains revealed that the regulatory inactivation of DnaA component Hda is necessary for maintaining controlled initiation but not for cell growth or viability.Keywords
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