Target accessibility dictates the potency of human RISC
- 24 April 2005
- journal article
- research article
- Published by Springer Nature in Nature Structural & Molecular Biology
- Vol. 12 (5) , 469-470
- https://doi.org/10.1038/nsmb931
Abstract
In this report, we examined the effect of increased target site access on activated human RNA-induced silencing complex (RISC*) catalysis. Kinetic studies revealed that siRNA-programmed RISC* cleaved target RNA with higher efficiencies when target site access was increased. These results provide evidence that target site access is linked to RISC* catalysis.Keywords
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