Three-dimensional structure of a specific pre-messenger RNP particle established by electron microscope tomography

Abstract
Electron microscope tomography has been used to refine the structural analysis of individual RNP particles synthesized on Balbiani ring genes in Chironomus tentans. The spherical particles have a diameter of 500 Å and are composed of a thick RNP ribbon bent into an asymmetrical, four-domain, ring-like configuration. The first domain, containing the 5′ end of the transcript, and the fourth domain, containing the 3′ end, are close to each other.