Sensitive radioimmunological screening test for antithyroglobulin autoantibodies.
Open Access
- 1 February 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 24 (2) , 272-274
- https://doi.org/10.1093/clinchem/24.2.272
Abstract
The test described here was designed as a screening test to be used in conjunction with the radioimmunoassay for human thyroglobulin. Anti-thyroglobulin autoantibodies, when present even in low concentrations, interfere with thyroglobulin determination. A 30-min incubation allows binding of 125I-thyroglobulin to endogenous anti-thyroglobulin autoantibodies. A subsequent 2-h incubation with goat anti-human gamma-globulin causes precipitation of such complexes. The immunoprecipitable radioactivity reflects the binding capacity of the sample for 125I-labeled thyroglobulin. Samples that are devoid of autoantibodies, and are therefore suitable for valid thyroglobulin determinations, bind less than 6% of the radiolabeled thyroglobulin. The sensitivity of this radioimmunoassay exceeds that of the tanned-erythrocyte hemagglutination test.This publication has 2 references indexed in Scilit:
- A practical radioimmunoassay of thyroglobulin.Clinical Chemistry, 1978
- Radioimmunoassay for Human Antithyroglobulin Antibodies of Different Immunoglobulin ClassesHormone and Metabolic Research, 1976