Phenylalanine as substrate for tyrosine hydroxylase in bovine adrenal chromaffin cells
- 1 June 1990
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 268 (2) , 525-528
- https://doi.org/10.1042/bj2680525
Abstract
Incubation of bovine chromaffin cells with L-[14C]phenylalanine resulted in label accumulation in catecholamines at about 30% of the rate seen with L-tyrosine as precursor. Studies with purified tyrosine hydroxylase (EC 1.14.16.2) showed that the enzyme catalysed the hydroxylation of L-phenylalanine first to L-p-tyrosine and then to 3,4-dihydroxyphenylalanine (DOPA). No evidence for a significant involvement of an L-m-tyrosine intermediate in DOPA formation was found.This publication has 19 references indexed in Scilit:
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