The analysis of terbutaline in biological fluids by gas chromatography electron impact mass spectrometry
- 1 January 1979
- journal article
- research article
- Published by Wiley in Journal of Mass Spectrometry
- Vol. 6 (1) , 31-37
- https://doi.org/10.1002/bms.1200060108
Abstract
A highly sensitive and specific assay is described for the bronchodilator drug terbutaline in human plasma and urine, based on single ion monitoring gas chromatography mass spectrometry and employing a homologue of the drug as internal standard. Recovery of terbutaline and internal standard into ethyl acetate is effected at pH 9.8, while back-extraction into dilute acid serves to purify the initial extract. Following preparation of O-TMS, N-TFA derivatives, the drug and its homologue are detected by selected ion monitoring of their common base ion at m/e 355. The limit of detection of terbutaline by this procedure is 0.3 ng ml−1 from a 4 ml sample of plasma.Keywords
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