A comparison of three tests for rubella antibody screening.

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Abstract
Sera from 1000 women attending antenatal clinics were screened for rubella antibody presence by hemagglutination-inhibition (HI), single radial hemolysis (SRH) and enzyme-linked immunosorbent assay (ELISA). With 925 sera, concordant results were obtained with all 3 tests. There were 46 false-negative HI tests due to necessity of allowing for residual non-specific inhibitors. With SRH there was 1 false positive and 3 that were negative by SRH but HI- and ELISA-positive. By ELISA 14 sera gave a false-positive result (OD 405 nm .gtoreq. 0.2). Of these, 10 could be eliminated by taking an OD 405 nm of 0.5 as the threshold; another 10 sera became false negatives.