Simultaneous Demonstration of Connective Tissue elastica and Fibrin by a Combined Verhoeff's Elastic-Martius-Scarlet-Blue Trichrome Stain

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Abstract
A method for the routine combined demonstration of elastica, connective tissue in general, and fibrin is described, Elastica, stained blue-black by Verhoeff''s iron hematoxylin, is contrasted with muscle and collagen, stained red and blue or green, respectively, by a modification of the Martius-scarlet-blue (MSB) trichrome for fibrin of Lendrum et al. The MSB technique selectively stains fresh, mature and old fibrin orange-yellow, red and blue, respectively; the Masson trichrome does not distinguish between erythrocytes and fibrin. Nuclei are stained at the same time as the elastica. The technique takes .apprx. 1.50 h and is ideal for the study of connective tissue and vascular pathology, especially the necrotizing vasculitides [in humans].