A Modified Hypotonic Treatment for Increasing the Frequency and Quality of Meiotic Metaphases from Spermatocytes of the Syrian Hamster

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Abstract
A modified hypotonic treatment for the spermatocytes of the Syrian hamster (M. auratus) was developed using a micro-osmometer to determine optimum conditions for the frequency and quality of Metaphase I and II figures. A suspension of germinal cells from the tubules is made in 3.2% sodium citrate solution and allowed to stand for 5 min. Water is then added until the concentration of the sodium citrate is reduced to 1.4%. This is followed after 15 min by fixation and air drying. The number and quality of the metaphase figures is enhanced considerably by this technique. A comparison is made of blood, plasma and testicular osmolality in several animal species [2 mouse strains, human, Sorex araneus] and corresponding values for various solutions used for cytogenetic techniques.