High-level expression and epitope localization of the major outer membrane protein of Chlamydia trachomatis serovar L1
- 1 September 1988
- journal article
- research article
- Published by Wiley in Molecular Microbiology
- Vol. 2 (5) , 681-685
- https://doi.org/10.1111/j.1365-2958.1988.tb00077.x
Abstract
Fragments of the gene encoding the major outer membrane porin protein (MOMP) of Chlamydia trachomatis serovar L1 were ligated into the pUC plasmid vectors to give a series of overlapping recombinant sex pressing MOMP from the lac promoter. Induction of this promoter with IPTG leads to high‐level expression of the recombinant porin protein. Electron microscopy shows the presence of insoluble inclusions within the Escherichia coli host cells. Probing the expressed MOMP fragments with a set of monoclonal antibodies permitted localization of the four binding sites (epitopes)of primary‐sequence‐dependent monoclonal antibodies that exhibit genus‐, species‐, subspecies and type (serovar)‐specific reactivities.This publication has 22 references indexed in Scilit:
- Epitope mapping with solid‐phase peptides: identification of type‐, subspecies‐, species‐ and genus‐reactive antibody binding domains on the major outer membrane protein of Chlamydia trachomatisMolecular Microbiology, 1988
- Sequence and transcriptional start site of the Pseudomonas aeruginosa outer membrane porin protein F geneJournal of Bacteriology, 1988
- Porin protein of Neisseria gonorrhoeae: cloning and gene structure.Proceedings of the National Academy of Sciences, 1987
- Identification of conserved regions for species and subspecies specific epitopes on the major outer membrane protein of Chlamydia trachomatisMicrobial Pathogenesis, 1987
- Computer prediction of potential immunogenic determinants from protein amino acid sequenceAnalytical Biochemistry, 1987
- Synthesis of disulfide-bonded outer membrane proteins during the developmental cycle of Chlamydia psittaci and Chlamydia trachomatisJournal of Bacteriology, 1986
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primersGene, 1982
- Chlamydial InfectionsAnnual Review of Medicine, 1981
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970