Transverse relaxation of saline and plasma using Mn(II), HSA–EDTA–Mn, and HSA–EDTA–Gd: Application to erythrocyte water exchange

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Abstract
Enhancements of 1H nuclear magnetic transverse relaxation rates by Mn(II)‐ and Gd(III)‐ethylenediaminetetraacetic acid (EDTA)‐human serum albumin (HSA) complexes are compared with MnCl2 in human plasma and isotonic saline. HSA–EDTA–Mn and HSA–EDTA–Gd in saline, at physiological concentrations of HSA, afforded T2's comparable to 2 mM MnCl2 in 4% HSA saline. The above reagents are new and potentially useful relaxation agents for the measurement of H2O exchange rates in erythrocyte suspensions. The exchange rate of water across the red cell membrane was determined using these new reagents over the temperature range from 20 to 37°C. © 1987 Academic Press, Inc.