Blue fluorescent proteins with enhanced brightness and photostability from a structurally targeted library
- 19 November 2006
- journal article
- research article
- Published by Springer Nature in Nature Biotechnology
- Vol. 24 (12) , 1569-1571
- https://doi.org/10.1038/nbt1264
Abstract
The utility of blue fluorescent protein (BFP) has been limited by its low quantum yield and rapid photobleaching. A library targeting residues neighboring the chromophore yielded a variant with enhanced quantum yield (0.55 versus 0.34), reduced pH sensitivity and a 40-fold increase in photobleaching half-life. This BFP, named Azurite, is well expressed in bacterial and mammalian cells and extends the palette of fluorescent proteins that can be used for imaging.Keywords
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