Differential Susceptibility to Herpes Simplex Viruses of Hamster Cell Lines Established after Exposure to Chemically Inactivated Herpesvirus

Abstract
The replication of herpes simplex virus type 1 and herpes simplex virus type 2 was studied in two isolated hamster cell lines. These lines developed after being treated with herpes simplex virus type 2 inactivated with 7,12-dimethylbenz(a)-anthracene. One cell line that developed, HDC-22, had a hypodiploid chromosome number and was missing a D-group chromosome. Growth studies revealed that this cell line did not support the replication of herpes simplex virus type 2 but did support the replication of herpes simplex virus type 1. The other cell line, HDC-17, had a normal karyotype and proved susceptible to both herpes simplex virus type 1 and herpes simplex virus type 2. These hamster cell lines afford an opportunity to study specific resistance to herpes simplex virus and the differences in the replicative cycles of type 1 and type 2 herpesviruses.

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