A Comparative Study of Dapi, Dipi, and Hoechst 33258 and 33342 as Chromosomal Dna Stains
- 1 January 1985
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 60 (1) , 7-11
- https://doi.org/10.3109/10520298509113885
Abstract
A comparison of four DNA stains considered to be AT-specific with chromosomes from a clonal Chinese hamster cell line B14F28-C5 have been made. The flow karyotype histograms indicate that DAPI, DIPI, and Hoechst 33258 and 33342 do stain similarly in the same preparation. DAPI staining is specific and highly reproducible in this line. We, therefore, recommend this dye as a single chromosome DNA stain for high-resolution flow cytometric measurements in cytogenetics and mutation research.This publication has 7 references indexed in Scilit:
- Cytochemical studies of metaphase chromosomes by flow cytometryChromosoma, 1980
- Flow cytogenetics of uncloned and cloned Chinese hamster cellsCytogenetic and Genome Research, 1980
- Rapid isolation of metaphase chromosomes containing high molecular weight DNA.The Journal of cell biology, 1979
- Spectral studies on 33258 Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of deoxyribonucleic acid synthesis.Journal of Histochemistry & Cytochemistry, 1976
- The Acid-Base Properties, Hydrolytic Mechanism, and Susceptibility to O 2 Oxidation of Fe 4 S 4 (SR) 4 -2 ClustersProceedings of the National Academy of Sciences, 1975
- Staining of constitutive heterochromatin in mammalian chromosomes with a new fluorochromeExperimental Cell Research, 1972
- Trypanocide Diamidine des 2‐Phenyl‐benzofurans, 2‐Phenyl‐indens und 2‐Phenyl‐indolsEuropean Journal of Organic Chemistry, 1971