EFFECT OF CHELATING-AGENTS ON IRON MOBILIZATION IN CHANG CELL-CULTURES

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  • 1 January 1976
    • journal article
    • research article
    • Vol. 48  (6) , 923-929
Abstract
The investigation of chelating agents with potential therapeutic value in patients with transfusional Fe overload was facilitated by the use of Chang cell cultures [human liver cells]. These cells were incubated with [59Fe]transferrin for 22 h, following which most of the intracellular radioFe is found in the cytosol, distributed between a ferritin and a nonferritin form. Fe release from the cells depends on transferrin saturation in the medium, but when transferrin is 100% saturated, which normally does not allow Fe release, desferrioxamine, 2,3-dihydroxybenzoic acid, rhodotorulic acid, cholylhydroxamic acid and tropolone all promote the mobilization of ferritin Fe and its release from cells. They are effective to an approximately equal degree. The incubation of [59Fe]transferrin with tropolone in vitro at a molar ratio of 1:500 results in the transfer of most of the labeled Fe to the chelator, reflecting the exceptionally high binding constant of this compound.

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