The Role of Secondary Messengers in the Regulation of Lipid Peroxidation in Rat Liver Microsomes
- 1 January 1988
- journal article
- research article
- Published by Taylor & Francis in Free Radical Research Communications
- Vol. 4 (5) , 277-281
- https://doi.org/10.3109/10715768809066892
Abstract
The effect of phorbol-12-myristate-13-acetate (PMA), an activator of protein kinase C (PK-C) on lipid peroxidation (LPO) in rat liver homogenates and microsomes was studied. PMA (10(-10) to 10(-6) M) produced a concentration-dependent inhibition of LPO, which was greatly decreased by polymyxin B (PxB) (an inhibitor of PK-C). The non-active analogue of PMA, 4 alpha-phorbol-12,13-didecanoate (4 alpha-PDD) exerted no inhibitory effect. The adenylate cyclase activator, forskolin (FK) (10(-6) M) abolished the inhibitory effect of PMA on LPO. PMA and FK did not inhibit LPO in liposomes. It is suggested that LPO in biomembranes could be regulated by PK-C, whose inhibitory effect might be prevented by cAMP-dependent protein kinases.Keywords
This publication has 15 references indexed in Scilit:
- Stimulation of soluble guanylate cyclase by endothelium-derived relaxing factor from cultured endothelial cellsEuropean Journal of Pharmacology, 1987
- Regulation of inositol trisphosphate accumulation by muscarinic cholinergic and H1-histamine receptors on human astrocytoma cells. Differential induction of desensitization by agonistsBiochemical Journal, 1987
- Inhibition of intercellular communication between mouse hepatocytes by tumor promoters*1, *2Toxicology and Applied Pharmacology, 1987
- Inhibition of calcium flux and calcium channel antagonist binding in the PC12 neural cell line by phorbol esters and protein kinase CBiochemical and Biophysical Research Communications, 1986
- The effects of polymyxin B, a protein kinase C inhibitor, on insulin secretion from intact and permeabilised islets of LangerhansBiochemical and Biophysical Research Communications, 1986
- Influence of lipid peroxidation on β‐adrenoceptorsFEBS Letters, 1986
- Toxic and Protective Effects of Antioxidants in BiomembranesPublished by Springer Nature ,1986
- Rapid effects of phorbol esters on isolated rat adipocytes. Relationship to the action of protein kinase CEuropean Journal of Biochemistry, 1985
- Alpha-adrenoceptor stimulation, lysophosphoglycerides, and lipid peroxidation in reoxygenation induced calcium uptake in rabbit myocardiumCardiovascular Research, 1985
- Activation of guanylate cyclase by superoxide dismutase and hydroxyl radical: a physiological regulator of guanosine 3',5'-monophosphate formation.Proceedings of the National Academy of Sciences, 1977