Mutagenicity and clastogenicity of acrylamide in L5178Y mouse lymphoma cells

1 January 1987

journal article
research article
Published by Wiley in Environmental Mutagenesis

Vol. 9 (3) , 261-267
https://doi.org/10.1002/em.2860090305

Abstract

Acrylamide was tested without exogenous activation in L5178Y/TK^+/− −3.7.2C cells for mutation at the thymidine kinase locus and for clastogenicity. Acrylamide gave a positive induced mutagenic response (approximately 70 mutants/10⁶ survivors) when tested at 600–650 μg/ml. The highest dose tested (850 μg/ml) resulted in an induced mutant frequency of approximately 380 mutants/10⁶ survivors (survival = 13%). Acrylamide induced almost exclusively small-colony mutants, indicating that it might be acting by a clastogenic mechanism. As predicted, acrylamide was clastogenic, inducing both chromatid and chromosome breaks and rearrangements. A clearly positive clastogenic response was observed at both the 750 μg/ml and 850 μg/ml doses, which showed 16 and 64 aberrations per 100 cells, respectively (background = 3 aberrations per 100 cells). These studies indicate that the L5178Y/TK^+/− mouse lymphoma assay can detect some chromosomal mutagens (clastogens) that show little activity in other single gene mutation assays, the CHO/HPRT and Salmonella.

Keywords

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