Influence of hemolysis on the serum lactate dehydrogenase-1/lactate dehydrogenase-2 ratio as determined by an accurate thin-layer agarose electrophoresis procedure.
Open Access
- 1 October 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 27 (10) , 1708-1711
- https://doi.org/10.1093/clinchem/27.10.1708
Abstract
The accuracy of our lactate dehydrogenase (EC 1.1.1.27) isoenzyme procedure, based on electrophoresis in thin-layer agarose, has previously been assessed (Clin. Chem. 22: 1995, 1976). We now show that the method is similarly accurate and precise for the specific measurements of lactate dehydrogenase isoenzyme-1 and -2. We have also determined the lactate dehydrogenase isoenzyme pattern in peripheral erythrocytes and have found that lactate dehydrogenase-1 is usually the isoenzyme present in greatest concentration. Therefore, hemolysis may increase lactate dehydrogenase-1 in serum, affecting the lactate dehydrogenase isoenzyme ratio used in the diagnosis of myocardial infarction. This conjecture has been confirmed by in vitro experiments, and in a small group of patients with intra-vascular hemolysis.This publication has 1 reference indexed in Scilit: