Analytical Gradient Polyacrylamide Gel-Crossed Line Immunoelectrophores. A Technique for Locating Species SpecificMycobacterium TuberculosisH37Rv Antigens in Polyacrylamide Gel Columns
- 1 January 1980
- journal article
- research article
- Published by Taylor & Francis in Immunological Communications
- Vol. 9 (6) , 595-609
- https://doi.org/10.3109/08820138009052998
Abstract
Analytical gradient polyacrylamide gel electrophoresis of antigens from Mycobacterium tuberculosis H37RV was combined with crossed line Immunoelectrophoresis to locate the position of antigens in polyacrylamide gel columns that appeared to be specific for M. tuberculosis H37RV. Specificity was established by comparing H37RV with M. kansasii CE in the electrophoretic procedure and the relative mobility (Rm) values are being used in an effort to recover and enrich specific antigens from preparative gel columns. Such fractions should be more suitable in efforts to recover purified monospecific components from M. tuberculosis H37RV.This publication has 5 references indexed in Scilit:
- Disc-crossed immunoelectrophoresis. A simple ‘laying-on’ technique permitting the use of commercially available agaroseJournal of Immunological Methods, 1976
- Gradient polyacrylamide gel electrophoresis of human serum proteins: Improved discontinuous gel electrophoretic technique and identification of individual serum componentsClinica Chimica Acta; International Journal of Clinical Chemistry, 1971