Sulfation of Phenolic Antibiotics by Sulfotransferase Obtained from a Human Intestinal Bacterium.

1 January 1992

journal article
Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN

Vol. 40 (4) , 1056-1057
https://doi.org/10.1248/cpb.40.1056

Abstract

Novel sulfotransferase which was isolated from Eubacterium A-44, a human intestinal bacterium, sulfated phenolic antibiotics, such as amoxicillin, ceaodroxil and cefoperazone. The Km values of sulfotransferase for these antibiotics were 6.9, 4.3 and 22.2 mM, respectively. The Vmax values were 8.3, 3.3 and 1.6 mumol/min/mg protein. The optimal pH of the enzyme was 9.0, a weakly alkaline region. The antibacterial activity of amoxicillin was not altered by enzymic sulfation of the phenolic hydroxyl group.

Keywords

OPTIMAL
SULFOTRANSFERASE
PROTEIN
SULFATED
HUMAN INTESTINAL
INTESTINAL BACTERIUM
PHENOLIC ANTIBIOTICS
ENZYMIC SULFATION

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