The Use of Pluronic Polyols in the Precipitation of Plasma Proteins and Its Application in the Preparation of Plasma Derivatives

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Abstract
Pluronic F-38 was used as a precipitant of plasma proteins under varying conditions of pH and polymer concentration. Results indicated that marked differences in the solubility of the plasma proteins in F-38 solutions can be applied to the separation of plasma components. The feasibility of the industrial application of this fractionation method was tested in several experiments. Conditions were established for the preparation of albumin, human plasma protein fraction (HPPF) and immune serum globulin (ISG) with similar yield and purity as those prepared by the Cohn method. Current procedures for the preparation of antihemophilic A (Factor VIII) concentrate and prothrombin complex (Factors II, VII, IX and X) were adapted to the F-38 process by removal of the clotting factors from the starting plasma prior to polymer precipitation. In addition, a plasma protein solution free of lipoproteins, isoagglutinins and clotting factors was developed which has proven useful as a perfusion medium in organ preservation.