"Protease I" of Escherichia coli functions as a thioesterase in vivo
- 1 March 1994
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 176 (6) , 1793-1795
- https://doi.org/10.1128/jb.176.6.1793-1795.1994
Abstract
Escherichia coli protease I is assayed as an esterase active with certain synthetic model chymotrypsin substrates. However, the gene encoding protease I has the same DNA sequence and genomic location as tesA, a gene that encodes E. coli thioesterase I. We report that both hydrolase activities utilize the same active site and demonstrate that the protein functions as a thioesterase in vivo.Keywords
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