Role of Mercaptoethanol and Endotoxin in Stimulating B Lymphocyte Colony Formation in Vitro
Open Access
- 1 March 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 116 (3) , 635-638
- https://doi.org/10.4049/jimmunol.116.3.635
Abstract
Mercaptoethanol is necessary to permit B lymphocyte colony formation in semi-solid agar cultures of cells from normal mouse lymphoid organs. Transfer studies on developing colonies showed that, in part, this was a direct action on B lymphocyte colony cells but evidence was produced that in the presence of mercaptoethanol lymphoid organ cells release a factor promoting colony growth. Endotoxin strongly potentiated B lymphocyte colony formation in vitro by a direct action on colony cells but in the absence of mercaptoethanol did not allow cell survival or proliferation.This publication has 0 references indexed in Scilit: