DNA Repair in Congenic Mice: Possible Influence of a Chromosome 4 Genetic Region on the Rate of Benzo[a]pyrene-Induced DNA Adduct Removal
- 1 January 1993
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Gerontology
- Vol. 48 (1) , B11-B16
- https://doi.org/10.1093/geronj/48.1.b11
Abstract
An attempt was made to assign mouse lifespan-associated interstrain differences in DNA repair to a specific chromosomal region using a set of congenic mice. The sensitive 32P-postlabeling assay was employed to measure the removal of benzo[a]pyrene-induced DNA adducts in liver DNA of three different chromosome 4 congenic mouse strains: B6.C-H-15C,B6.C-H-16C, and B6.C-H-26C and the two parental strains, C57B1I6 and BALB/c. The removal of the one main adduct detected, trans-(7R)-N2-[10-(7β,8α,9α-trihydroxy)-7,8,9,10-tetrahydrobenzo(a)-pyrene]-yldeoxyguanosine (BPDE-N2-dG), in liver DNA of C57B1I6 and BALBIc mice between one and three days after treatment, was approximately 86% and 57%, respectively. The percentage removal of BPDE-N2-dG in two of the three congenic mouse strains, B6.C-H-16C and B6.C-H-26C, resembled that found in BALBIc, whereas the third strain, B6.C-H-15C, removed about the same amount as C57B1I6, i.e., approximately 88% of BPDE-N2-dG between one and three days after treatment. The usefulness ofcongenic mouse strains for identifying genes putatively involved in aging and/or disease susceptibility is discussed.Keywords
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