Signaling by Toll-Like Receptor 2 and 4 Agonists Results in Differential Gene Expression in Murine Macrophages
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Open Access
- 1 March 2001
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 69 (3) , 1477-1482
- https://doi.org/10.1128/iai.69.3.1477-1482.2001
Abstract
Lipopolysaccharide (LPS) derived from the periodontal pathogenPorphyromonas gingivalishas been reported to differ structurally and functionally from enterobacterial LPS. These studies demonstrate that in contrast to protein-free enterobacterial LPS, a similarly purified preparation ofP. gingivalisLPS exhibited potent Toll-like receptor 2 (TLR2), rather than TLR4, agonist activity to elicit gene expression and cytokine secretion in murine macrophages and transfectants. More importantly, TLR2 stimulation by thisP. gingivalisLPS preparation resulted in differential expression of a panel of genes that are normally induced in murine macrophages byEscherichia coliLPS. These data suggest that (i)P. gingivalisLPS does not signal through TLR4 and (ii) signaling through TLR2 and through TLR4 differs quantitatively and qualitatively. Our data support the hypothesis that the shared signaling pathways elicited by TLR2 and by TLR4 agonists must diverge in order to account for the distinct patterns of inflammatory gene expression.Keywords
This publication has 49 references indexed in Scilit:
- Toll-Like Receptors Confer Responsiveness to Lipopolysaccharide fromPorphyromonas gingivalisin Human Gingival FibroblastsInfection and Immunity, 2000
- Bacterial Lipopolysaccharide Activates NF-κB through Toll-like Receptor 4 (TLR-4) in Cultured Human Dermal Endothelial CellsJournal of Biological Chemistry, 2000
- CD14 Employs Hydrophilic Regions to “Capture” LipopolysaccharidesThe Journal of Immunology, 2000
- Toll-like receptor 4 imparts ligand-specific recognition of bacterial lipopolysaccharideJournal of Clinical Investigation, 2000
- Host Defense Mechanisms Triggered by Microbial Lipoproteins Through Toll-Like ReceptorsScience, 1999
- Escherichia coli and Porphyromonas gingivalis Lipopolysaccharide Interactions with CD14: Implications for Myeloid and Nonmyeloid Cell ActivationClinical Infectious Diseases, 1999
- Endotoxin-tolerant Mice Have Mutations in Toll-like Receptor 4 (Tlr4)The Journal of Experimental Medicine, 1999
- Elimination of trace endotoxin protein from rough chemotype LPSInnate Immunity, 1994
- Characterization of the lipopolysaccharide from the polymyxin‐resistant pmrA mutants of Salmonella typhimuriumFEBS Letters, 1981
- Chemical, Physical, and Biological Properties of a Lipopolysaccharide from Escherichia coli K-235*Biochemistry, 1967