Characterization of muscarinic receptor subtypes inhibiting Ca2+ current and M current in rat sympathetic neurons.
- 15 October 1992
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 89 (20) , 9544-9548
- https://doi.org/10.1073/pnas.89.20.9544
Abstract
Muscarinic receptors mediating suppression of Ca2+ current and of M-type K+ current in rat superior cervical ganglion neurons were subclassified pharmacologically by using the muscarinic receptor antagonists pirenzepine and himbacine. Our voltage clamp experiments previously distinguished fast and slow intracellular signaling pathways coupling muscarinic receptors to calcium channels. We now establish that the fast, pertussis toxin-sensitive suppression of Ca2+ current is mediated primarily by muscarinic receptors of the M4 subtype, whereas the slow, bis(2-aminophenoxy)-ethane-N,N,N',N'-tetraacetate (BAPTA)-sensitive suppression of Ca2+ current is mediated primarily by muscarinic receptors of the M1 subtype. Both actions on Ca2+ current are blocked by guanosine 5'-[beta-thio]diphosphate. Muscarinic suppression of M current is slow, BAPTA-sensitive, and mediated by receptors of the M1 subtype. Hence the two muscarinic pathways use different receptors and different guanine nucleotide binding proteins to produce different actions on channels.Keywords
This publication has 43 references indexed in Scilit:
- L-type voltage-sensitive calcium channels mediate synaptic activation of immediate early genesNeuron, 1991
- Genotypic m3‐Muscarinic Receptors Preferentially Inhibit M‐currents in DNA‐transfected NG108‐15 Neuroblastoma × Glioma Hybrid CellsEuropean Journal of Neuroscience, 1991
- Muscarinic suppression of the M-current is mediated by a rise in internal Ca2+ concentrationNeuron, 1991
- Selective coupling of different muscarinic acetylcholine receptors to neuronal calcium currents in DNA-transfected cellsProceedings Of The Royal Society B-Biological Sciences, 1990
- Muscarinic Receptor SubtypesAnnual Review of Pharmacology and Toxicology, 1990
- Inhibition of Ca2+ and K+ channels in sympathetic neurons by neuropeptides and other ganglionic transmittersNeuron, 1990
- Elementary properties and pharmacological sensitivities of calcium channels in mammalian peripheral neuronsNeuron, 1989
- Primary structure of porcine muscarinic acetylcholine receptor III and antagonist binding studiesFEBS Letters, 1988
- M-currents: an updateTrends in Neurosciences, 1988
- Primary structure of porcine cardiac muscarinic acetylcholine receptor deduced from the cDNA sequenceFEBS Letters, 1986