Theileria -mediated constitutive expression of the casein kinase II-α subunit in bovine lymphoblastoid cells

Publisher Website
Google Scholar
Abstract
Theileria-infected cells are induced to undergo a transformation that is reversible, since their proliferation is inhibited after elimination of the schizonts by the theilericidal drug buparvaquone. The molecular mechanisms of the transformation remain unknown. The experiments described in the present report deal with the role of casein kinase (CK) II, a serine/threonine protein kinase, in the permanent proliferation of the parasitized cells and show that the CK II-α subunit is expressed in both T. annulata- and T. parva-infected cells and that its expression is closely related to the presence of the parasites in the host-cell cytoplasm. Thus, elimination of the schizonts by buparvaquone leads to the inhibition of CK II-α subunit mRNA expression without affecting the expression of actin. Cells treated with 5,6-dichloro-1-β-d-ribofuranosylbenzimidazole (DRB) are inhibited in a dose-dependent manner from undergoing DNA synthesis as measured by [3H]-thymidine incorporation and from expressing CK II. Furthermore, a host-cell-specific CK II-α antisense inhibits DNA synthesis in a dose-dependent manner. In the present study, 6 μM antisense reduced [3H]-thymidine incorporation by Theileria-infected bovine cells to about 50 %. Using a primer derived from T. parva CK II, we detected a parasite-specific CK II mRNA in T. parva-infected cell lines. Interestingly, DRB also inhibited the expression of the parasite-specific CK II. However, to date we have not detected a target sequence for this primer in T. annulata schizonts.

This publication has 0 references indexed in Scilit: