Follicle-Stimulating Hormone and Luteinizing Hormone Content of Pooled Human Menopausal Plasma and of Subfractions Prepared by Cohn Methods 6 and 9
- 1 May 1964
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 24 (5) , 425-431
- https://doi.org/10.1210/jcem-24-5-425
Abstract
Pooled human menopausal plasma was fractionated according to a modification of Cohn''s methods 6 and 9 and assayed for FSH and LH activity against a human menopausal urine preparation, Pergonal 23, employed as a standard. The Steelman-Pohley method was employed for the estimation of FSH and the method of Greep et al. for the estimation of LH content. FSH activity was demonstrable in all the Cohn subtractions but was concentrated mainly in Fractions V and VI. LH activity was likewise demonstrable in all the subtractions but was concentrated mainly in Fraction n+m. The indices of discrimination for these fractions depart sufficiently from unity to suggest that a partial separation of the 2 gonadotropic hormones has been accomplished.Keywords
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