cDNA clones encoding IgE-binding factors from a rat-mouse T-cell hybridoma.
- 1 April 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (8) , 2460-2464
- https://doi.org/10.1073/pnas.82.8.2460
Abstract
C[complementary]DNA clones encoding rodent IgE-binding factors (IgE-BF) were isolated from cDNA libraries of a rat-mouse T hybridoma that secretes IgE-suppressive factor (IGE-SF) upon incubation with rat IgE. COS7 [chicken] cells transfected with 2 of the cDNA expressed IgE-BF, which selectively potentiate an in vitro IgE response. IgE-BF expressed in COS7 cells are glycoproteins of .apprxeq. 60 and .apprxeq. 11 kDa [kilodalton]. DNA sequence analysis of an IgE-BF cDNA revealed a 556-amino acid (62 kDa) protein coding region. Evidently, IgE-potentiating and IgE-suppressive factors share common precursor polypeptides and the 11-kDa IgE-BF is derived from a 6-kDa precursor.This publication has 25 references indexed in Scilit:
- The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primersGene, 1982
- Formation of rat IgE-binding factors by rat-mouse T cell hybridomas.The Journal of Immunology, 1982
- Regulatory role of IgE-binding factors from rat T lymphocytes. V. The carbohydrate moieties in IgE-potentiating factors and IgE-suppressive factors.The Journal of Immunology, 1982
- Nucleotide sequence of Moloney murine leukaemia virusNature, 1981
- Mouse beta 2-microglobulin cDNA clones: a screening procedure for cDNA clones corresponding to rare mRNAs.Proceedings of the National Academy of Sciences, 1981
- SV40-transformed simian cells support the replication of early SV40 mutantsCell, 1981
- Regulatory role of IgE-binding factors from rat T lymphocytes. II. Glycoprotein nature and source of IgE-potentiating factor.The Journal of Immunology, 1980
- Regulatory role of IgE-binding factors from rat T lymphocytes. III. IgE-specific suppressive factor with IgE-binding activity.The Journal of Immunology, 1980
- Regulatory role of IgE-binding factors from rat T lymphocytes. I. Mechanism of enhancement of IgE response by IgE-potentiating factor.The Journal of Immunology, 1980
- Lymphocytes bearing Fc receptors for IgE. IV. Formation of IgE-binding factor by rat T lymphocytes.The Journal of Immunology, 1980