T-cell cloning to detect the mutant 6-thioguanine-resistant lymphocytes present in human peripheral blood.
- 1 November 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (21) , 6617-6621
- https://doi.org/10.1073/pnas.79.21.6617
Abstract
Rare thioguanine-resistant T lymphocytes, present in vivo in human peripheral blood, were isolated and grown in vitro as thioguanine-resistant cultured T cells. The conditions for their selection in vitro were such that thioguanine resistant had to have arisen in vivo. The mutant cells bore T-cell surface markers, maintained their thioguanine resistance in vitro in the presence or absence of selection, and were deficient in hypoxanthine-guanine phosphohribosyltransferase activity.Keywords
This publication has 19 references indexed in Scilit:
- TCGF Production for Cloning and Growth of Functional Human T LymphocytesScandinavian Journal of Immunology, 1980
- Enumeration of 6-thioguanine-resistant peripheral blood lymphocytes in man as a potential test for somatic cell mutations arising in vivoMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1979
- Phenotypic lag and mutation to 6-thioguanine resistance in diploid human lymphoblastsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1978
- Selective Systems in Somatic Cell GeneticsPublished by Springer Nature ,1976
- Resistance of cultured human fibroblasts and other cells to purine and pyrimidine analogues in relation to mutagenesis detectionMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1974
- Variability of the time at which PHA-stimulated lymphocytes initiate DNA synthesisExperimental Cell Research, 1973
- Precipitation of radioactively labeled samples: A semi-automatic multiple-sample processorCellular Immunology, 1972
- The spontaneous azaguanine-resistant mutants of diploid human fibroblastsPublished by Springer Nature ,1972
- Genetic studies of HG- PRT deficiency and the Lesch-Nyhan syndrome with cultured human cells.1971
- Enzyme Defect Associated with a Sex-Linked Human Neurological Disorder and Excessive Purine SynthesisScience, 1967