Separation of cholesterol, and fatty acylglycerols, acids and amides by thin‐layer chromatography

Abstract
A rapid unidimensional thin‐layer chromatographic (TLC) method for the separation of neutral lipids is described, using two sequential solvent systems of different polarity. Excellent separations of mono‐, di‐ and triglycerides, fatty acids, fatty amides, and cholesterol are thereby achieved. Separation is accomplished at room temperature and requires 25 min.