EFFECTS OF AMILORIDE ON CONTRACTIONS AND THE RELEASE OF TRITIUM FROM RAT VAS DEFERENS PRELOADED WITH [3H]‐NORADRENALINE

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Abstract
1 The effect of amiloride was studied on contractions and tritium release from rat vas deferens preloaded with [3H]-noradrenaline 2 Amiloride had no effect on the resting tension and maximal contractile force of the vas deferens and did not alter the ED50 of noradrenaline 3 Amiloride (10−4-10−3m) decreased the response of vas deferens to electrical stimulation dose-dependently without inhibiting the response to KC1 (60 mm) 4 The effect of amiloride was not prevented by preincubation of the tissue with phentolamine, propranolol, atropine or indomethacin 5 Amiloride did not alter the spontaneous outflow of radioactivity from [3H]-noradrenaline labelled vasa deferentia 6 Amiloride decreased the release of tritium induced by electrical stimulation or nicotine but did not inhibit the release of radioactivity induced by KC1 or tyramine. 7 It is concluded that amiloride may inhibit the contractions of rat vas deferens by inhibiting the release of noradrenaline.