X-Ray Analysis and Circular Dichroism of the Acid Protease from Endothia Parasitica and Chymosin

Abstract
Central to the study of mechanism and specificity of the acid proteinases is a knowledge of the three-dimensional structures of enzymes with different physiological roles. The availability of acid proteinases with either extracellular or intracellular roles in vertebrates as well as similar enzymes from plants, protozoa, and fungi allows a wide range of comparative studies. In our laboratory we have undertaken the x-ray analysis of fungal enzymes, those from Endothia parasitica and Mucor pusillus and some vertebrate enzymes including chymosin and chicken pepsin, and are beginning work with cathepsin D.