Freeze-fracturing of Pseudomonas phaseolicola Infected by the Lipid-containing Bacteriophage 6

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Abstract
The freeze-fracturing of the lipid-containing bacteriophage φ6 shows the virus membrane to have structural asymmetry with smooth convex and rough concave fracture faces. The host, Pseudomonas phaseolicola, shows the four typical fracture faces of the Gram-negative cell envelope. During the infection of P. phaseolicola by φ6 the virus membrane and the bacterial outer membrane fuse to form a bulge. The nucleocapsid obviously also penetrates the cytoplasmic membrane and is seen inside the cell shortly after infection. The outer membrane of the host undulates and after infection small membrane vesicles are formed apparently from the outer leaflet of the outer membrane.