An HPLC Procedure for the Analysis of Furosemide in Pharmaceuticals-Analysis of Furosemide Tablets and Furosemide Injection

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Abstract
A simple and specific procedure was developed for the analysis of furosemide from tablets and injections. The procedure consists of extracting furosemide into aqueous sodium hydroxide, addition of the internal standard, appropriate dilution and injection onto a u Bondapak C18 reversed phase column. The mobile phase consisted of a solvent containing acetonitrile and aqueous sodium acetate and the eluate was monitored by either U.V. absorption or spectrofluorimetry. A standard linear calibration curve was obtained for direct standard solutions containing 75 ng to 500 ng on column. This procedure was successfully used to analyze furosemide tablets (individual assay) and injections.