Phosphorylation of D‐glyceraldehyde‐3‐phosphate dehydrogenase by Ca2+/calmodulin‐dependent protein kinase II
- 25 April 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 231 (2) , 413-416
- https://doi.org/10.1016/0014-5793(88)80861-5
Abstract
Rabbit muscle D-glyceraldehyde-3-phosphate dehydrogenase was shown to serve as a substrate for Ca2+/calmodulin-dependent protein kinase II with a K m of 0.33 μM and a V max of 2.63 μmol · min−1 · mg−1 at pH 7.5 and 30°C. In the absence of calmodulin, the V max was halved and K m unchanged. 0.99 mol of phosphate was incorporated per tetrameric molecule of D-glyceraldehyde-3-phosphate dehydrogenase under the experimental conditions employed.Keywords
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