Continuous-flow chemiluminescence determination of some corticosteroids

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Abstract
A continuous-flow method for the determination of cortisone, hydrocortisone, prednisolone and methylprednisolone in the range 0.100–1.00 µg ml–1 and of dexamethasone and betamethasone in the range 0.500–5.00 µg ml–1 is described. The procedure is based on the action of each corticosteroid as a sensitiser on the chemiluminescent oxidation of sulphite by cerium(IV). The analysis is automated, requires no sample pre-treatment and samples can be analysed at a rate of 40 per hour with a relative error of <6%. The method was evaluated by carrying out recovery experiments and by analysing commercial formulations. Results agreed well with those given by an official method.

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