Differential Effects of Two Interferon‐Induced Translational Inhibitors on Initiation of Protein Synthesis
Open Access
- 1 May 1979
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 96 (1) , 35-41
- https://doi.org/10.1111/j.1432-1033.1979.tb13010.x
Abstract
At least two different mechanisms for the inhibition of mRNA translation operate in extracts of interferon-treated L cells. One is mediated by an interferon-induced protein kinase which, when activated by double-stranded RNA and ATP, phosphorylates the small subunit of initiation factor eIF-2. Addition of the purified interferon-induced protein kinase to L cell extracts, strongly reduces the amount of methionyl-tRNA bound to 40-S ribosomal subunits. The second translational inhibition is due to the synthesis of (2′-5′)oligo(adenylate) by interferon-induced enzyme E. The oligonucleotide in turn activates a ribonuclease F constitutively present in L cells. Addition of the purified nuclease with its oligonucleotide activator to L cell extracts produces a strong decrease in polyribosome formation and an accumulation of initiation complex. These experiments differentiate the effects of the two interferon-induced inhibitors on mRNA translation.This publication has 26 references indexed in Scilit:
- Interferon action: Isolation of Nuclease F, A translation inhibitor activated by interferon‐induced (2′–5′) oligo‐isoadenylateFEBS Letters, 1978
- Interferon action may be mediated by activation of a nuclease by pppA2′p5′A2′p5′ANature, 1978
- Dual Action of Double‐Stranded RNA in Inhibiting Protein Synthesis in Extracts of Interferon‐Treated Mouse L CellsEuropean Journal of Biochemistry, 1978
- Interferon, double-stranded RNA and RNA degradation. Fractionation of the endonucleaseINT system into two macromolecular components; Role of a small molecule in nuclease activationBiochemical and Biophysical Research Communications, 1978
- Mechanism of interferon action partial purification and characterization of a low-molecular-weight interferon-mediated inhibitor of translation with nucleolytic activityBiochemical and Biophysical Research Communications, 1977
- Synthesis of low molecular weight inhibitor of protein synthesis with enzyme from interferon-treated cellsNature, 1977
- Interferon, double-stranded RNA and mRNA degradationNature, 1976
- Specific phosphorylation in vitro of a protein associated with ribosomes of interferon‐treated mouse L cellsFEBS Letters, 1976
- Blocks in Elongation and Initiation of Protein Synthesis Induced by Interferon Treatment in Mouse L CellsEuropean Journal of Biochemistry, 1975
- A complex between Met-tRNAF and native 4OS subunits in reticulocyte lysates and its disappearance during incubation with double-stranded RNABiochemical and Biophysical Research Communications, 1972