Reversed-Phase Separations of Subcomponents of Minor Soybean Phospholipids--Glycerol Esters of Phosphatidic Acids

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Abstract
A reversed-phase ion-pair high-performance liquid chromatographic (HPLC) technique was developed to resolve molecular species of phosphatidyl glycerol and diphosphatidyl glycerol. Homologues of quaternary ammonium phosphates (QAP) were used as counter ions in a solvent system of acetonitrile-methanol-water. Retention characteristics of the lipid analytes were dramatically affected by varying the concentration and the structural type of QAP. An increase in the concentration and size of QAP resulted in proportional enhancements in solute retention. The HPLC results are indicative of an ion-pair retention mechanism during the separation processes. Structures of counter ions appeared to have some influence on the availability of the total area of QAP for solvophobic interactions.

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