Effects of Trypsin on Glucose-Induced Insulin Secretion from the Perfused Rat Pancreas

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Abstract
Glucose-induced insulin secretion was significantly inhibited by preperfusion with trypsin (10 .mu.g/ml) in the isolated rat pancreas perfusion. However, when trypsin was interposed during perfusion of 16.6 mM glucose, glucose-induced insulin secretion was not suppressed during trypsin infusion. The insulin level was significantly lower in the trypsin treated animals than in the control after completion of trypsin infusion. Trypsin probably does not attack the glucoreceptor bound with glucose but attacks the free glucoreceptor. A biphasic pattern of glucose-induced insulin secretion disappeared by the pretreatment with trypsin. Trypsin may have prevented the initial binding of glucose to the .beta.-cell.