Interaction of difluoro-oxaloacetate with aspartate transaminase
- 1 February 1977
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 161 (2) , 383-387
- https://doi.org/10.1042/bj1610383
Abstract
Diffluoro-oxaloacetate behaves as a competitive inhibitor of 2-oxoglutarate and as an uncompetitive inhibitor with respect to aspartate in steady-state kinetic experiments with cytoplasmic aspartate transaminase. In the presence of high concentrations of aspartate transaminase, difluoro-oxaloacetate is slowly transaminated to difluoro-aspartate, suggesting its use as a kinetic probe to study the reactions of the aminic form of the enzyme.This publication has 8 references indexed in Scilit:
- The direct linear plot. A new graphical procedure for estimating enzyme kinetic parametersBiochemical Journal, 1974
- The complete amino acid sequence of cytoplasmic aspartate aminotransferase from pig heartFEBS Letters, 1973
- Use of multivariate non-linear regression analysis in fitting enzyme kinetic modelsJournal of Theoretical Biology, 1969
- On the formation of S-(α,β-dicarboxyethyl) derivatives of glutamic-aspartic aminotransferaseBiochemical and Biophysical Research Communications, 1964
- INTERACTION BETWEEN BETA-FLUORO-OXALOACETATE AND GLUTAMATE-ASPARTATE TRANSAMINASE OF HEART MITOCHONDRIA1960
- GLUTAMIC ASPARTIC TRANSAMINASE .1. ASSAY, PURIFICATION, AND GENERAL PROPERTIES1959