Effects of Some Monovalent Anions on Fluxes of Na and K, and on Glucose Metabolism of Ouabain Treated Human Red Cells

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Abstract
The present work deals with the effects of the anions Cl, Br, NO3, I, SCN, and HCO3 on Na and K fluxes, and on glucose metabolism of human red cells suspended in isotonic electrolyte media at 38d̀ C and pH 7.40 in the presence of ouabain. When 120 m M Cl of the isotonic medium was substituted by the anion concerned, Na influx and K efflux were unaffected by Br, increased about 30 per cent by NO3 and I, but increased 200 to 250 per cent in the presence of SCN. Substitution of 120 mM Cl by HCO3 increased Na influx into red cells by 250 per cent. In contradistinction K efflux was reduced by 25 per cent. The increase of Na influx was linearly related to HCO3 concentration at pH 7.40.At intracellular Na concentrations between 10 and 35 meq/kg cell water the rate constant of Na efflux was close to 0.15 h‐1 in the presence of all 6 anions studied. At external K concentrations from 5 to 7.6 meq/l potassium influx into ouabain treated cells suspended in a chloride medium was thrice as big as the influx calculated from the flux ratio equation M1/M0=K0/K1. exp (‐zEF/RT). A similar condition was found in the presence of Br. In all other media studied (NO3.l, SCN, and HCO3) the ratio between the measured fluxes of potassium agreed with the calculated values of flux ratio for passive, independent fluxes.A net flux of water into the cells was observed in cells suspended in SCN and HCO3 media. In the same media the gain of Na in cells exceeded K loss. The relation of water net flux to the net gain of cations was 6.5–7.3 g water per meq. The rate of glycolysis was unaffected by the substitution of Cl by HCO3, was reduced by 15 per cent in the presence of Br, NO3, and I, and decreased by 30 per cent in the presence of SCN.

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