Malformations induced in cultured rat embryos by enzymically generated active oxygen species

1 January 1986

journal article
research article
Published by Wiley in Teratogenesis, Carcinogenesis, and Mutagenesis

Vol. 6 (6) , 547-554
https://doi.org/10.1002/tcm.1770060608

Abstract

Day 9.5 rat embryos were exposed in culture to xanthine/xanthine oxidase generated active oxygen species. Growth and development were assessed after 46 hr of culture. The treatment induced abnormalities of the neural suture, the severity of which increased in a dose‐related manner with the concentration of substrate or enzyme. Glutathione (10 mM) or catalase (50 μg/ml) either partially or completely abolished the effects of xanthine/xanthine oxidase, whereas the addition of superoxide dismutase (50 μg/ml) or desferrioxamine (1mM) did not reduce the number of malformed embryos. These findings suggest that hydrogen peroxide and/or hydroxyl radicals are responsible for the effects of xanthine and xanthine oxidase.

Keywords

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