Cultures of Rat Anterior Pituitary Cells with Differential Gonadotrophin Secretion and Tinctorial Properties12

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Abstract
The question of whether LH and FSH are secreted by the same or different rat anterior pituitary cells was investigated in vitro. Clone cultures were initiated from single cells in individual microwells and the levels of LH and FSH secreted into the medium were measured by radioimmunoassay. Media from 41% of clones contained only FSH, and that from 4.3% of clones contained only LH. Media from 1.7% of the wells contained both hormones. The presence of both LH and FSH seems most likely due to chance seeding of these wells with multiple cells. Media from 53% of clones contained no gonadotropins. The tinctorial characteristics of the cell clones, following staining with PAS—Methyl Blue— Orange G (Rennels, E. G., Z Zellforsch 45: 464, 1957) were correlated with the nature of the secreted hormones. Clones which secreted only LH stained PAS—red and clones which secreted only FSH stained PAS—purple. Clones which secreted no gonadotropins were usually PAS—negative and occasionally PAS—red. These results indicate the existence of two physiologically and tinctorially distinct classes of gonadotrophs in rat anterior pituitary, each concerned with the secretion of a single hormone.(Endocrinology92: 6, 1973)

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