SV40-transformed human fibroblasts exhibit a characteristic chromosomal pattern

Publisher Website
Google Scholar
Abstract
A karyotype study of seven SV40-transformed human fibroblast cell lines was performed using R-banding. Although large variations existed from line to line and, to a lesser degree, from cell to cell in a given line, many common features were found. The most characteristic were chromosome imbalances. Some of the chromosomes or chromosome segments present in excess were, in decreasing order of frequency, the early replicating X, 12q, 3q, 12p, 19, 1p, and 6p. Losses of other chro mosomes and chromosome segments were also frequent and involved lip, 2p, 6q, 10 p, 18, 4q, 8p, 4p, 10q, and 16. These imbalances seemed to correlate with metabolic characteristics, previously described, such as low activities of catalase (lip), superoxide dismutase 2 (6q), and acid phosphatase (2p, lip) and a high ratio of lactate dehydrogenase B (LDHB, 12p) activity to LDHA (lip) activity.

This publication has 11 references indexed in Scilit: